Figure 1.

MAX binds 5caC DNA. (A–E) Electrophoretic mobility shift assay of full length MAX protein binding to oligonucleotides containing a single E-box where the central CpG are unmodified (C/C), fully modified (M/M, H/H, F/F), or hemi-modified (C/M, C/H, C/F or C/5caC). The protein concentrations used were a maximum of 0.5 μM (the right most lane of each panel) followed by serial 2-fold dilutions (from right to left). (F) DNA binding was quantified by fluorescence polarization. Data represent the mean ± SEM of two independent determinations performed in duplicate.