Fig 9. B. bronchiseptica in amoeba sori can be transmitted via an intermediary to a new geographical location.

(A) Schematic showing the experimental setup in which flies were added to conical tubes containing B. bronchiseptica–filled fruiting bodies. Flies were then transferred to new plates containing either a lawn of K. pneumoniae (yellow plates, top) to assess amoeba transmission or Bordet-Gengou (BG) agar with streptomycin (red plates, bottom) to assess Bordetella transmission. (B) Representative image showing amoeba plaque growth along the fly’s footprints. (C) Representative image showing B. bronchiseptica colony growth along the fly’s footprints. Bb, B. bronchiseptica; Dd, D. discoideum; Kp, K. pneumoniae.