Figure 1.

Sub1 interacts with the RNAPII through the Rpb4/7 heterodimer. (A) Sub1 tandem affinity purification from Sub1–TAP and Sub1–TAP Rpb7–HA whole cell extracts (WCE). Input and the purified proteins were precipitated with trichloroacetic acid and analyzed by western blotting using anti-TAP and anti-HA. Anti-Pgk1 was used as a loading control. (B) Co-IP performed using WCEs from Sub1–TAP (Rpb7–HA and Rpb7–HA/Rpb4–HA) with IgG Sepharose. Input and IPs were analyzed by western blotting with antibodies to the indicated proteins.