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Sub1 and Fcp1 are components of the same complex. (A) Co-IP performed on WCEs from Sub1–HA and Sub1–HA Fcp1-MYC cells using an anti-HA antibody. Inputs and IPs were analyzed by Western blotting with antibodies to the indicated proteins (anti-MYC and anti-HA for Fcp1 and Sub1, respectively). (B) Same as in (A), except that in the fourth IP the beads were washed with lysis buffer containing 500 mM NaCl, and second and third IPs were treated with DNAse I and RNAse A, respectively.
