FIG. 1.

CDT-V causes G₂/M arrest in human endothelial cells. (A) Flow cytometric analysis of HUVEC, EA.hy 926 cells, and HBMEC after 1 and 5 days of treatment with 8 CD₅₀ of CDT-V/ml or with the control preparation. The proportions of cells in G₁ (2n DNA) and G₂/M (4n DNA) are indicated. Black arrowheads indicate the sub-G₁ population. Data represent one of three independent experiments. (B) HUVEC, EA.hy 926 cells, and HBMEC were treated with 1 (triangles), 2 (squares), or 8 (circles) CD₅₀ of CDT-V/ml or with the control preparation (diamonds) for the indicated times. The proportions of cells in G₂/M were determined by flow cytometry. Time zero indicates basal numbers of cells in G₂/M, which were obtained by exposing the cells to CDT-V or the control preparation for 5 min, followed by immediate flow cytometric analysis. Data are means and standard deviations from three independent experiments.