FIG. 4.
CDT-V induces the accumulation of phosphorylated, inactive cdc2 kinase. EA.hy 926 cells and HBMEC were treated with 8 CD50 of CDT-V/ml (lanes 2 and 5), the vector preparation (lanes 1 and 4), or nocodazole (lanes 3 and 6) for 4 h (lanes 1 to 3) or 8 h (lanes 4 to 6). Isolated total cellular proteins were analyzed by immunoblotting with anti-cdc2 antibody. The strong signals displayed by cells treated with CDT-V (lanes 2 and 5) were double bands which were not completely separated on the gels.