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RarA binds double-stranded DNA in the presence of ATPγS and single-stranded DNA in the absence of nucleotide cofactor. RarA binding affinity for dsDNA (A) and ssDNA (B) was measured using florescence polarization in the presence of various nucleotide cofactors. A table of calculated dissociation constants for each reaction condition is provided in (C). Error bars represent one standard deviation from the mean polarization value.
