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Application of four-plex MS-NaME (ATM, RARb2, MGMT and GSTP1 gene promoters) on bisulfite-treated DNA to enrich methylated or unmethylated DNA. A 50:50 methylated: unmethylated DNA mix was used and results were assessed via MS-HRM (A) or sequencing (B). The MS-HRM curves are as follows: No-DSN control-green, U-antisense probes-blue, and M-antisense probes-red.
