Figure 2.

Gene annotation facilitated by RNA-seq and peptide evidence. Screenshot from the WebApollo genome annotation editor showing a locus where RNA-seq and peptide evidence improved gene annotation compared to the previous annotation described by Gioti et al. (5). The 5΄-UTR and protein-coding segments were identified by the MAKER-based pipeline integrating RNA-seq and peptide data. Manual curation added a 3΄-UTR (uppermost track). The colors of exons and peptides indicate reading frame, such that exons and peptides with the same color are in the same reading frame. UTRs are indicated in purple and introns in gray. RNA-seq coverage is shown for the genomic minus strand (i.e. the strand of the annotated gene) and indicates the number of read pairs at each base.