Table 1. M. sympodialis nuclear chromosome sizes and predicted centromeres.

Chr	Size in current assembly (bp)	Size estimate from PFGE (Mb)	Putative centromeric region (CEN)	GC content of 250 bp trough	Size of CEN (bp)
1	1 508 930	1.55	786 541–787 061	16.4%	520
2	1 381 175	1.37	355 760–355 841	20.0%	81
3	1 353 702	1.37	237 534–238 686	15.6%	1152
4	1 203 350	1.17	418 202–418 728	15.2%	526
5	709 412	0.75	125 056–125 220	18.0%	164
6	634 681	0.62	101 950–102 502	14.4%	552
7	517 958	0.53	431 542–431 987	13.2%	445
8	438 251	0.47	24 694–25 564	18.4%	870
Total	7 747 459	7.83	n.a.	n.a.	4310

The PFGE karyotyping and corresponding chromosome size estimates have been described previously (6,66). Note that bands for chromosome 2 and 3 overlapped in the PFGE gel. n.a., not applicable.