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. 2017 Apr 12;12(4):e0174968. doi: 10.1371/journal.pone.0174968

Fig 6. PEDF receptors expression in vitro.

Fig 6

(A) Total RNAs from RAW 264.7 and BMDMs (tumor-free mice) were analyzed by qRT-PCR and normalized to S15. Results are presented as relative fold change compared to ATP5B mRNA expression levels in macrophages. Data points represent mean ± SD of triplicate samples from two independent experiments. Statistical analyses were performed using the Student’s t test, *: p < 0.05. (B) Expression levels of ATP5B, PNPLA2, and LRP6 mRNAs in RAW 264.7 cells and BMDMs collected from tumor-free and tumor-bearing mice, quantified by qRT-PCR and normalized to S15 mRNA. Data points represent mean ± SD of triplicate samples from two independent experiments. Error bars denote ± SD (*p < 0.05). (C) Western blot showing the expression levels of ATP5B and PNPLA2 in RAW 264.7 cells treated with PEDF (10 nM), P18 (10 nM) or control. Actin is used as a loading control. (D) Quantification of ATP5B and PNPLA2 protein levels averaged from three different experiments. Error bars show standard error of the mean. *: P <0.05. (E) ATP5B (Red) and PNPLA2 (Green) immunostaining of RAW 264.7 macrophages and BMDMs collected from tumor-bearing mice. Macrophages were treated with PEDF (10 nM), P18 (10 nM) or buffer control. Nuclei are visualized by DAPI (Blue) staining.