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. 2017 Feb 1;45(5):2644–2657. doi: 10.1093/nar/gkx011

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Cellular localization of PALB2 mutant proteins. (A) Immunofluorescence of mutant forms of Flag-tagged PALB2. DAPI (blue), anti-Flag (red), and the merge picture are shown. All mutants except W1038X were mainly nuclear. (B) Quantification of the cytoplasmic and nuclear accumulation of PALB2 mutants. Experiments were performed in quadruplicate. (C) Knockdown of endogenous PALB2 in HEK293T cells by expressing constitutively a shRNA against PALB2. (D) Analysis of the cellular localization of PALB2 mutant forms by cellular fractionation. The cytoplasmic (C), nuclear soluble (NS) and chromatin (N) fractions are shown. Blots against the GAPDH and histone H3 proteins are shown as controls.