Table 1. Distribution of recombination/mutation events obtained after spontaneous marker loss.
| APRT (−)*** | GPT (−)*** | HSV-TK (−) | APRT (−)/HSV-TK (−) | |||||
|---|---|---|---|---|---|---|---|---|
| RAD51D-WT n (%) | RAD51D (−) n (%) | RAD51D-WT n (%) | RAD51D (−) n (%) | RAD51D-WT n (%) | RAD51D (−) n (%) | RAD51D-WT n (%) | RAD51D (−) n (%) | |
| STGC | 20 (30%) | 1 (2%) | 1 (3%) | 0 (0%) | NA | NA | NA | NA |
| LTGC | 5 (8%) | 0 (0%) | 3 (10%) | 0 (0%) | NA | NA | NA | NA |
| SSA | 19 (29%) | 21 (42%) | 13 (43%) | 12 (40%) | 11 (29%) | 14 (38%) | 41 (98%) | 38 (97%) |
| Deletion | 4 (6%) | 11 (22%) | 3 (10%) | 18 (60%) | 13 (34%) | 14 (38%) | 1 (2%) | 1 (3%) |
| Othera | 18 (27%) | 17 (34%) | 10 (33%) | 0 (0%) | 14 (37%) | 9 (24%) | 0 (0%) | 0 (0%) |
| N | 66 | 50 | 30 | 30 | 38 | 37 | 42 | 39 |
aOther indicates small mutations to coding sequence, such as point mutations, frameshifts, and small indels that do not change the overall structure of the region and are not readily discernable by Southern blot or PCR analysis.
***Indicates significant difference (P < 0.001) in endpoint spectra between RAD51D-WT and RAD51D(−) cells for that particular selection pressure as determined by Fisher's exact test.
NA—not applicable.