Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 26;45(4):2040–2050. doi: 10.1093/nar/gkw854

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — In vivo binding of tRNA^fMet mutants to the ribosomes. (A) Total ribosomal cell-free extracts from KL16ΔmetY containing plasmid borne metY genes (wild-type, wt; or mutants) were separated on 15–35% sucrose gradient and their profiles were analyzed at 254 nm. The tRNAs from 30S, 50S and 70S fractions were separated on native PAGE and analyzed by northern blotting using a probe specific to tRNA^fMet. The metZWV encoded tRNA^fMet1 was used as internal control (shown by arrowhead). All tRNA^fMet mutants were derived from tRNA^fMet2 and indicated by asterisk. (B) Quantification of fractional distribution of tRNA^fMet2 (wt or mutants) within ribosomal subunits and 70S. Individual tRNA intensities were divided with the total tRNA^fMet intensity in the corresponding profile to obtain the fractional distribution.