Figure 5.

Mutation of Lysine 311 causes global changes to the LNCaP transcriptome. (A) RNA was sequenced in triplicate from LNCaP cells expressing WT or K311R AR and genes most significantly deregulated in the presence of DHT were plotted. (B) RNA was sequenced in triplicate from LNCaP cells expressing WT or K311R AR and genes most significantly deregulated in the absence of DHT were plotted. (C-D) Venn diagram of genes up- (C) and down- (D) regulated in K311R expressing LNCaP cells compared to WT AR expressing cells. (E) Table of metabolic pathways most significantly affected by mutation in K311 of the androgen receptor.