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Density-based separation of protein complexes by glycerol gradient centrifugation. (A) Protein–protein complexes were separated by glycerol gradient centrifugation. Precipitated proteins were then resolved in SDS-PAGE and analyzed by Western blotting. α-SKI2W, α-DIS3L and α-HBS1L antibodies were used. (B) SKI2W and DIS3L band intensities are shown as a line graph.
