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. 2016 Sep 20;45(4):2007–2015. doi: 10.1093/nar/gkw839

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Mass spectral region for the RNase A fragment AGGGGAACp from the loop of 16S rRNA helix 45 (nucleotides 1513–1520) in the E. coli derivatives. The number of methyl groups added to this sequence is indicated at the bottom of the figure. (A) The wild-type E. coli rRNA fragment at m/z 2761 is stoichiometrically modified with five methyl groups. (B) The spectrum from the E. coli rsmA-null mutant contains a single methyl group at m²G1516 in the m/z 2705 peak, and this pattern remained unchanged upon expression of NEQ337. (C) The E. coli rsmA-null mutant expressing Igni_1059 gave rise to a series of peaks from mono- and dimethylation intermediates at A1518 and A1519.