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. 2016 Dec 2;45(4):2124–2136. doi: 10.1093/nar/gkw1120

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Detection of 2-methylthio-cyclic-N⁶-threonylcarbamoyladenosine (ms²ct⁶A). (A) The chemical structure of ms²t⁶A (left) and ms²ct⁶A (right). The 2-methylthio modification is shown in red, and the N⁶-threonylcarbamoyl group and its hydantoin form are shown in blue. (B) Secondary structures of B. subtilis tRNA^Lys (left) and T. brucei tRNA^Lys (right) with post-transcriptional modifications: dihydrouridine (D), 5-carboxymethylaminomethyl-2-thiouridine (cmnm⁵s²U), 2-methylthio cyclic N⁶-threonylcarbamoyladenosine (ms²ct⁶A), pseudouridine (Ψ), 7-methylguanosine (m⁷G), 5-methyluridine (m⁵U), N²-methylguanosine (m²G), 3-(3-amino-3-carboxypropyl)uridine (acp³U), N², N²-dimethylguanosine (m^2,2G), 2΄-O-methylcytidine (Cm), 5-methoxycarbonylmethyl-2-thiouridine (mcm⁵s²U), 3-(3-amino-3-carboxypropyl)dihydrouridine (acp³D), 5-methylcytidine (m⁵C). The position numbers of the residues are displayed according to the nucleotide numbering system (44). Pairs of gray triangles in B. subtilis tRNA^Lys indicate the positions of cleavage by RNase T₁ that generate RNA fragments containing the anticodon region.