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. 2016 Dec 2;45(4):2124–2136. doi: 10.1093/nar/gkw1120

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Detection of ms²ct⁶A in plant tRNAs. HILIC/ESI-MS nucleoside analyses of total RNAs from N. tabacum, S. oleracea and A. thaliana. Top panels show UV traces at 254 nm. Panels second from the bottom show mass chromatograms corresponding to the proton adducts of t⁶A (m/z 413), ms²t⁶A (m/z 459), ct⁶A (m/z 359) and ms²ct⁶A (m/z 441), respectively. Unassigned peaks are indicated by asterisks. Conversion of adenosine to inosine observed in spinach and A. thaliana is due to the contamination of adenosine deaminase activity in PDase II (Sigma P9041).