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. 2016 Dec 2;45(4):2124–2136. doi: 10.1093/nar/gkw1120

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Detection of ms²ct⁶A in T. brucei tRNAs. (A) RPC/ESI-MS nucleoside analyses of total RNAs from T. brucei. Top panel shows a UV trace at 254 nm. Panels second from the bottom show mass chromatograms corresponding to the proton adducts of t⁶A (m/z 413), ms²t⁶A (m/z 459), ct⁶A (m/z 395) and ms²ct⁶A (m/z 441), respectively. (B) Mass chromatograms showing the proton adduct of ms²ct⁶A (m/z 441) in total nucleosides of B. subtilis (top panel), T. brucei (middle panel) and the co-injection fraction (bottom panel) obtained by RPC/ESI-MS. (C) CID spectrum of BH₂⁺ of ms²ct⁶A from T. brucei. The parent ion for CID is indicated by an arrow.