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. 2016 Dec 6;45(4):2150–2165. doi: 10.1093/nar/gkw1215

Figure 1.

Figure 1.

NRP1 accumulation at the chromatin from CPT-treated A. thaliana cells. (A) Immunodetection of γ-H2AX (16 kDa) in total lysates of MM2d A. thaliana cells treated with 20 μM CPT for 0.5, 1 and 4 h. α-Tub (50 kDa) antibody was used as loading control. (B and C) Detection of NRP1 (38 kDa) in nuclei (B) or in the cytosolic fractions (C) of non-treated or CPT-treated-cells (20 μM CPT) for 4 h. (D) Chromatin fractionation and immunoblotting detection of NRP1 in the soluble nuclear and chromatin-associated fractions of non-treated and CPT-treated cells (20 μM) for 4 h. Specific antibodies against A. thaliana NRP1 and γ-H2AX were used in Western blotting. Antibodies against histone H3 (15 kDa) and α-Tub were used as loading nuclear and cytosolic controls, respectively.