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. 2016 Nov 29;45(5):2423–2437. doi: 10.1093/nar/gkw1187

Figure 3.

Figure 3.

Identification of the binding sites for SerRS and YY1 located at the upstream distal region of human vegfa. (A) Schematic illustrating the binding sites for SerRS and YY1 from −5868 to +55. The arbitrary amplicon sites were indicated on the bottom. (B) ChIP assay combined with PCR to analyze SerRS and YY1 binding sites. Immunoprecipitated DNA by anti-SerRS, anti-YY1 or control IgG from HEK293T cell lysates was amplified by PCR. A positive band was revealed for both SerRS ChIP and YY1 ChIP shown on amplicon 2. Input represents genomic DNA extract from HEK293T used for PCR analysis with 3 amplicon site primers. (C) ChIP-qPCR was used to quantify the total input of chromatin DNA at SerRS and binding sites. The amount of DNA immunoprecipitated by anti-SerRS, anti-YY1 antibodies and control IgG from HEK293T cell lysates was measured by qPCR on amplicons 1, 2 and 3. The amplicon 2 site located at the distal region of vegfa exhibited the highest expression. Results represent the percentages of total input of chromatin DNA. Data represent mean ± SD (n = 3).