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Diagram of asd-based expression plasmids pCFAI and pCFAI/LTB. (A) Map of pCFAI. The DNA fragment encoding the CfaA and CfaB genes was inserted at the NcoI and HindIII restriction sites of pYA3098. (B) Map of pCFAI/LTB. The DNA fragment encoding the CfaA, CfaB, and LTB genes was inserted at the NcoI and HindIII restriction sites of pYA3098. 5ST1T2 is a transcriptional terminator. Maps include approximate locations of restriction sites, genes, and the origin of replication.
