Figure 5.

Deleting RMI1 OB2 fold domain and TOP3α Zn finger motifs does not alter protein–protein interactions between the different members of the BTR complex. (A) Schematic representation of the different fusion proteins used for the yeast two hybrid assays. (B) Yeast two-hybrid assays. Interaction between the bait fused with the GAL4 DNA binding domain (pGAD) and the pray fused with the GAL4 activation domain (pGBK) is revealed by growth on media depleted of histidine (-His). Three replicates are shown for each test. Untested combinations are indicated by n.d.