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. 2016 Dec 6;45(4):1879–1888. doi: 10.1093/nar/gkw1228

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Rad52 and HsRAD52 associate with a DSB undergoing repair by EGCWhole cell extracts prepared from cells containing the EGC assay components and expressing Rad52-FLAG or HsRAD52-FLAG were collected before and various times after induction of HO-endonuclease and subjected to ChIP using anti-FLAG antibody. Immunoprecipitated and input DNA was probed for sequences adjacent to the DSB at the HIS3 locus or uncut sequences at the SAM1 locus by qPCR. Fold changes in degree of occupancy by Rad52-FLAG or HsRAD52-FLAG at the HIS3 locus relative to the SAM1 locus were normalized to those of a control strain lacking FLAG-tagged proteins and plotted against time after the initiation of the expression of HO endonuclease. Relevant strain genotypes are indicated in the legend. Strains used in this analysis: Untagged – ABX3834-2D; RAD52-FLAG – ABX3844-23D; rad51Δ RAD52-FLAG – ABX3879-17B; rad52Δ HsRAD52-FLAG – ABX3885-17B; rad51Δ rad52Δ HsRAD52-FLAG – ABX3885-32C.