FIG. 5.

PMT does not induce tolerance to the coadministered antigen OVA. Groups of six BALB/c mice were orally immunized two times with OVA alone, CT plus OVA, PMT plus OVA, or CT plus PMT plus OVA 2 weeks apart as described in Materials and Methods. Four weeks after the second immunization, all mice were immunized i.p. with 100 μg of OVA and 100 μg of KLH in CFA. Two weeks later, mice were bled, and anti-OVA endpoint titers (A) and anti-KLH endpoint titers (B) in the isolated sera of individual mice were determined by ELISA. The means and standard errors of the means are shown.