TABLE 4.
Inhibition of antibody binding to AMA1 constructs with homologous and heterologous allelic formsa
| AMA1 construct | Maximum inhibition (%)b | Concn for 50% OD405c | ||
|---|---|---|---|---|
| Full ectodomain
|
||||
| Coating 3D7 | ||||
| 3D7 | 100 | 0.204 | ||
| D10 | 98.1 ± 1.6 | 0.135 | ||
| HB3 | 83.8 ± 3.8 | 0.312 | ||
| Coating D10 | ||||
| 3D7 | 90.7 ± 3.2 | 0.338 | ||
| D10 | 100 | 0.207 | ||
| HB3 | 83.2 ± 4.0 | 0.492 | ||
| Coating HB3 | ||||
| 3D7 | 87.8 ± 4.7 | 0.530 | ||
| D10 | 98.1 ± 2.6 | 0.230 | ||
| HB3 | 100 | 0.381 | ||
| Domain III
|
||||
| Coating 3D7 | ||||
| 3D7 | 100 | 0.255 | ||
| D10 | 100.5 ± 2.0 | 0.223 | ||
| HB3 | 100.2 ± 2.1 | 0.238 | ||
| Coating D10 | ||||
| 3D7 | 98.8 ± 1.5 | 0.288 | ||
| D10 | 100 | 0.287 | ||
| HB3 | 97.8 ± 2.1 | 0.254 | ||
| Coating HB3 | ||||
| 3D7 | 98.7 ± 4.1 | 0.436 | ||
| D10 | 101.5 ± 2.1 | 0.284 | ||
| HB3 | 100 | 0.325 | ||
a
Values correspond to inhibition ELISA experiments performed with 13 (full ectodomain) or 5 (domain III) different sera, all of which exhibited similar recognition of the different allelic forms in direct ELISA experiments.
b
Percent inhibition (interpolated from the inhibition curve) obtained with 40 μg of inhibitor/ml relative to inhibition with the same concentration of the homologous form (the allelic form used for the coating). Values are the averages with 95% confidence intervals.
c
Concentration of inhibitor to reduce the OD405 in the absence of inhibitor by 50%. Values are the geometric means.