FIG. 8.
Effect of IL-1ra on ICAM-1 and IL-8 upregulation by rMsps. THP-1 cells (1 × 105 cells/ml) were cultured overnight. After the medium was replaced with serum-free medium, the cells were treated simultaneously with IL-1ra (50 ng/ml) and rMspA (10 μg/ml) or rMspTL (10 μg/ml) for 12 h. The ICAM-1 expression of the cells was analyzed by fluorescence-activated cell sorting (A). The supernatants were assayed for IL-8 by ELISA (B). The experiments were repeated three times, and representative means and standard deviations for mean fluorescence intensity (M.F.I) for ICAM-1 and means and standard deviations of triplicate values for IL-8 are shown. IL-1β (300 pg/ml) was used as a positive control. An asterisk indicates that the value is significantly different (P < 0.05) than the value obtained for the culture without IL-1ra. None, nontreated control.