FIG. 2.

hd-LBP inhibits binding of R and S LPS chemotypes to sCD14. Binding of R-type LPS from E. coli F515 (Re-LPS) (A), R-type LPS from S. enterica serovar Typhimurium TV 119 (Ra-LPS) (B), and S-type LPS from E. coli O111:B4 (C) to recombinant human sCD14 and the modulating effects of increasing concentrations of LBP were analyzed by native PhastGel electrophoresis. Following incubation at 37°C for 10 min, samples were subjected to automated native electrophoresis on 20% PhastGels and silver staining by using the PhastSystem apparatus. The positions of uncomplexed sCD14, sCD14-LPS complexes, LBP, and the LPS preparations are indicated. Arrows indicate different sCD14-LPS complexes containing most likely LPS of different chain lengths (C). As a loading control, the native samples were subsequently adjusted to a final concentration of 2% (wt/vol) SDS and additionally analyzed by PhastGel SDS electrophoresis and silver staining (lower panels).