Fig. 4. Comparison of site-specific amide backbone ¹⁵N NMR chemical shifts for the Notch-TMD as a function of the lipid used to form the bicelles in each of the three samples.

This plot shows the differences between the observed chemical shifts for each site minus the random coil chemical shift value for that amino acid. DMPC has C₁₄ chains. ESM has one C₁₆ chain as part of ceramide and a second usually C₁₆ chain at the fatty amide position. MSM has the ceramide C₁₆ and a usually C₂₂-to-C₂₄ chain at the fatty amide position.