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. 2005 Jan;73(1):70–78. doi: 10.1128/IAI.73.1.70-78.2005

FIG. 2.

FIG. 2.

Induction of Lig expression and release by tissue culture medium. (A) L. kirschneri RM52 was grown to late log phase. Samples of the culture were mixed with an equal volume of EMJH or tissue culture medium, which consisted of MEM with 10% FBS. The leptospires were incubated for 24 h, and the leptospires and culture supernatant were examined for Lig protein by immunoblot analysis. c, cells;su, culture supernatant. (B) L. kirschneri RM52 was grown as described for panel A, and samples of the culture were mixed with an equal volume of EMJH (lanes 1 and 2), MEM with 10% FBS (lanes 3 and 4), MEM (lanes 5 and 6), or 10% FBS (lanes 7 and 8). The cultures were incubated for 24 h. The leptospires and culture supernatant were examined for Lig protein by immunoblot analysis as for the experiment in panel A. (C) L. interrogans Fiocruz L1-130 was grown to late log phase. The bacteria were mixed with an equal volume of EMJH or MEM and incubated for 24 h. The leptospires and culture supernatant were examined for Lig protein by immunoblot analysis as for the experiment in panel A.