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. 2005 Jan;73(1):70–78. doi: 10.1128/IAI.73.1.70-78.2005

FIG. 6.

FIG. 6.

Sensitivity of Lig expression and release to sodium chloride concentrations. L. interrogans Fiocruz L1-130 grown in EMJH was collected by centrifugation and resuspended in EMJH (lanes 1 and 2) or EMJH containing an additional 40 mM NaCl (lanes 3 and 4), 80 mM NaCl (lanes 5 and 6), or 120 mM NaCl (lanes 7 and 8). The cultures were incubated for 24 h and examined for Lig expression and release following separation of proteins on an SDS-PAGE gel (10% polyacrylamide) gel. c, cells; s, culture supernatant fluid.