Fig 8. Effect of TCTP RNAi in the regeneration.
(A) Non injected control worm formed the regeneration blastema on the 3rd day. (B) The 6 nM GFP siRNA administrated worms were formed the regeneration blastema on the 3rd day. (C) The 6 nM TCTP siRNA administrated worms shows no regeneration blastema on the 3rd day. (D) The non-injected control animals reform the lost segments on the 5th day. Similarly, (E) The 6 nM GFP control siRNA injected worm normally continued the regeneration process. (F) The 6 nM concentration of TCTP siRNA injected worm on the 5th day, shows TCTP siRNA suppress the regeneration process. (G) Histological image (4X) of non injected worm tissue, it shows well developed regenerated part. (H) Histological image (4X) of 5th day GFP siRNA injected worm developed regeneration blastema normally as expected. (I) Histological image (4X) of 5th day TCTP siRNA injected worm tissue. It failed to form the regeneration blastema.