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. 2017 Apr 12;12(4):e0175336. doi: 10.1371/journal.pone.0175336

Fig 1. NLR expression in human PBMCs, monocytes and macrophages.

Fig 1

(A) mRNA gene expression was measured by RT-qPCR and presented as relative fold change of PBMCs. Data represent the mean ± standard error of the mean (SEM) of ≥ 4 experiments performed in duplicate in cells isolated from ≥ 4 independent donors. Mono: monocytes; Macro: macrophages. Asterisks indicate significant differences as compared to PBMCs (Mann Whitney test: * p < 0.05; ** p < 0.01). (B) Western blot analysis in total lysates of human PBMCs, monocytes and macrophages using anti-NLRP3 antibody. GAPDH was used as loading control. The blot shown is representative of at least 5 experiments from independent donors. Mono: monocytes; Macro: macrophages.