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. 2017 Apr 12;12(4):e0175396. doi: 10.1371/journal.pone.0175396

Fig 4. Neither overpressure nor incubation time showed significant main effects on area fraction of GFAP immunoreactivity.

Fig 4

Mean ± s.e.m area fraction of GFAP immunoreactivity as a function of incubation time. N within the bars indicates the slices used to collect the data. Each slice contributed to an average of 25 image fields. Main effects (overpressure, incubation) and interactions were tested in a general linear model (SAS Proc GLM) and none were significant. Post hoc comparison P values denote significant differences between bar pairs, or bars linked by horizontal line.