FIG. 2.

Noncytotoxic variant forms of ExoU are stably expressed in yeast and secreted by P. aeruginosa. A. Immunoblot analysis of cell lysates of yeast expressing noncytotoxic variants of ExoU. pVector refers to yeast containing empty vector. See Table 2 for an explanation of mutant ExoU designations. The lower band represents a yeast protein that is recognized by the anti-ExoU antiserum. B. Immunoblot analysis to detect wild-type and mutant ExoU secreted into supernatants of P. aeruginosa cultures.