FIG. 5.

Regions 1 to 5 of ExoU are required for cytotoxicity in HeLa cells. HeLa cells were transiently transfected with constructs encoding GFP or GFP-ExoU fusion proteins. Cytotoxicity was assessed by measuring LDH release into the culture medium 24 h after transfection and is expressed as the percentage of cells lysed. R1 to R5 refer to regions 1 to 5, respectively, and indicate the region in which each representative mutation is located. Error bars represent standard errors of the means.