TABLE 3.
Site-directed mutagenesis of individual residues within regions 1 to 5a
| Region 1 | Region 2 | Region 3 | Region 4 | Region 5 |
|---|---|---|---|---|
| T105G | R136L | Q278W | T340G | K601F |
| S106L | S137L | I279G | E341L | V602G |
| L107D | M138D | K280F | F342G | A603W |
| V108W | S139L | T281W | Q343W | V604W |
| L109D | G140W | L282D | D344A | I605G |
| S110L | S141L | N283F | G345W | A606W |
| G111W | S142A | I284G | G346W | E607W |
| G112W | S142L | T285W | V347G | N608F |
| G113W | A143W | G286W | M348G | I609G |
| A114W | G144W | T287W | I349G | R610F |
| K115A | G145W | A288W | N350F | K611F |
| K115F | I146G | V351G | E612W | |
| G116W | P352W | V613G | ||
| A117W | V353G | I614G | ||
| F615G | ||||
| P616W | ||||
| S617L | ||||
| L618G | ||||
| Y619G | ||||
| R620L |
a
Each mutation is designated as the wild-type amino acid, the location of the amino acid, and the amino acid to which it was changed. For example, T105G represents a change of the threonine at residue 105 to a glycine. Mutations resulting in a loss of cytotoxicity are shown in boldface type.