Table 2. Distribution of Cryptosporidium species by age groups according to genetic sequencing of fecal samples from 64 heads of cattle and PCR-RFLP of fecal samples from 15 heads of cattle from 28 dairy farms in Paraná, Brazil, from 2012 to 2014.
| Age Group (months) | DNA sequencing (%) | PCR-RFLPb Cryptosporidium spp. (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive Samples (nPCRa) | C. parvum | C. ryanae | C. bovis | ND c | Fragments of overlap | C. parvum/C. bovis | C. parvum/C. ryanae | C. parvum/C. andersoni | Single species | ND | |
| 0–2 | 33 | 16 | 0 | 0 | 4 | 13 | 2 | 2 | 0 | 5d | 4 |
| 2–4 | 8 | 4 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 |
| 4–6 | 8 | 4 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| 6–12 | 3 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1e | 0 |
| 12–24 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| >24 | 12 | 2 | 3 | 1 | 3 | 3 | 0 | 0 | 3 | 0 | 0 |
| Total | 64 | 27 (42.2) | 5 (7.7) | 2 (3.1) | 10 (15.6) | 20 (31.3) | 2 (3.1) | 3 (4.7) | 5 (7.8) | 6 (9.4) | 4 (6,3) |
anPCR: nested PCR,
bPCR-RFLP: restriction fragment length polymorphism,
c ND: species not determined (ND) due to illegible sequence (sequencing data) or absence of DNA (PCR-RFLP),
dC. parvum,
eC. bovis.