TABLE 2.
Genes regulated at least threefold by P. chabaudi infection or testosterone in spleen or liver
| Gene name | UNIPROTa | Spleen
|
Liver
|
||
|---|---|---|---|---|---|
| Day 8 p.i./day 0b (%) | Day 8 p.i. −T/ day 8 p.i. +Tc (%) | Day 8 p.i./day 0 (%) | Day 8 p.i. −T/ day 8 p.i. +T (%) | ||
| Cytokines, chemokines, growth factors, and their receptors | |||||
| Interleukin 10 (IL-10) | P18893 | 16.35/32 | 1.21/24 | ||
| Tumor necrosis factor alpha (TNF-α) | P06804 | 0.89/9 | 0.90/8 | 22,26/85 | |
| Gamma interferon (IFN-γ) | P01580 | 8.89/7 | 0.75/4 | ||
| Interleukin 1 receptor type II | P27931 | 5.26/15 | 1.22/4 | ||
| Cytokine receptor common β chain | P26955 | 1.38/5 | 0.95/4 | 3.50/6 | 1.42/11 |
| Lymphptoxin α | P09225 | 0.23/24 | 0.86/- | ||
| Lymphptoxin β | P41155 | 0.15/6 | 0.57/7 | ||
| C-C chemokine receptor 1 | P51675 | 2.20/6 | 0.79/5 | 10.88/82 | |
| Chemokine (C-C motif) ligand 2; MCP-1 | Q9QYD7 | 5.83/13 | 0.69/2 | 7.00/40 | |
| Chemokine (C-C motif) ligand 4; MIP-1β | P14097 | 8.75/14 | 0.89/5 | 6.05/32 | |
| Chemokine (C-C motif) ligand 5; RANTES | P30882 | 1.08/3 | 1.04/5 | 6.19/8 | 1.08/8 |
| Chemokine (C-C motif) ligand 6; MRP-1 | Q99M24 | 5.57/9 | 0.86/9 | 14.02/71 | 1.52/- |
| Chemokine (C-X-C motif) ligand 1; GRO1 | P12850 | 8.83/37 | 2.12/- | ||
| Chemokine (C-X-C motif) ligand 10; γ-IP10 | P17515 | 4.78/6 | 0.66/4 | 10.97/12 | 4.32/9 |
| Chemokine (C-X-C motif) ligand 9; MIG | P18340 | 11.80/10 | 1.04/13 | 30.62/24 | 0.77/10 |
| Growth differentiation factor 3; GDF3 | Q07104 | 4.38/8 | 1.73/7 | ||
| Bone morphogenetic protein 11; BMP11 | Q9QX55 | 0.14/9 | 0.66/- | ||
| Cysteine-rich angiogenic inducer 61; Cyr61 | P18406 | 0.41/7 | 0.51/9 | 4.49/8 | 8.99/16 |
| Insulin-like growth factor binding protein 1 | P47876 | 12.06/16 | 19.30/23 | ||
| Insulin-like growth factor 1 | P05018 | 1.04/5 | 1.09/5 | 0.25/6 | 0.85/4 |
| ECMd components and regulators; coagulation | |||||
| Tissue inhibitor of metalloproteinases 1 | P12032 | 3.02/4 | 0.80/11 | ||
| Matrix metalloproteinase-13 | P33435 | 20.96/19 | 1.60/5 | 16.68/15 | 2.57/7 |
| Syndecan 3 | Q64519 | 1.23/4 | 0.58/5 | 4.08/6 | 1.11/12 |
| Osteopontin | P10923 | 3.60/- | 0.70/7 | 0.63/5 | 0.82/13 |
| Plasminogen activator inhibitor; Serpine 1 | P22777 | 2.07/9 | 1.21/20 | 106.45/57 | 14.22/22 |
| Urokinase-type plasminogen activator receptor; CD87 | P35456 | 0.86/10 | 1.03/6 | 8.40/43 | |
| Coagulation factor F5 | O88783 | 0.39/3 | 0.25/6 | 1.12/18 | |
| Latent transforming growth factor-β-binding protein | Q8K4G1 | 0.09/8 | 0.70/5 | 0.18/37 | |
| GTPases | |||||
| IFNγ-inducible 47 kDa GTPase IF147, IRG-47 | Q61635 | 5.33/1 | 1.07/8 | 4.25/5 | 0.55/9 |
| IFNγ-induced isoprenylated 67-kDa GTPase; MGBP-2; GBP2 | Q9Z0E6 | 13.85/1 | 1.14/4 | 12.16/7 | 1.05/7 |
| IFNγ-induced GTPase IFII | Q60766 | 5.54/11 | 1.14/5 | 2.71/14 | 0.59/7 |
| IFNγ-induced GTPase IGTP | Q9DCE9 | 7.61/4 | 1.17/2 | 5.45/5 | 0.56/18 |
| IFNγ-induced GTPase IIGP | Q9Z1M3 | 7.89/21 | 0.93/9 | 1.79/8 | 0.50/7 |
| IFNγ-induced GTPase TGTP | Q60711 | 6.32/10 | 1.10/3 | 15.19/10 | 0.52/3 |
| Signal transduction proteins and transcription factors | |||||
| Protein kinase C-ɛ | P16054 | 0.40/6 | 1.04/14 | 0.26/- | |
| B lymphoid kinase | P16277 | 0.25/8 | 1.31/4 | ||
| Suppressor of cytokine signaling 1 | O35716 | 2.61/7 | 0.50/4 | 11.35/16 | 0.71/6 |
| Suppressor of cytokine signaling 2 | O35717 | 1.95/1 | 1.09/13 | 0.23/6 | |
| Suppressor of cytokine signaling 3 | P97803 | 2.18/4 | 0.64/4 | 5.51/12 | 1.45/10 |
| Dual-specificity protein phosphatase 1; MKP1 | P28563 | 2.14/2 | 3.28/11 | 2.75/5 | 6.41/10 |
| Dual-specificity protein phosphatase 2; PAC1 | Q05922 | 0.66/5 | 1.32/9 | 5.83/20 | 1.34/2 |
| NFIL3/E4BP4 transcription factor | O08750 | 3.30/16 | 1.14/10 | 1.86/9 | 2.27/12 |
| Activating transcription factor 3 | Q60765 | 4.02/12 | 0.74/6 | 18.97/25 | 7.05/19 |
| JunB oncogene; junB | P09450 | 0.48/3 | 0.48/3 | 3.38/4 | 3.10/7 |
| Fos-related antigen 2 | P47930 | 1.49/6 | 0.52/9 | 3.50/13 | 2.59/12 |
| Avian reticuloendotheliosis viral oncogene-related B (relB) | Q04863 | 0.33/10 | 0.70/6 | ||
| NFκB p49/p100 | Q9WTK5 | 0.32/9 | 0.65/12 | 1.79/18 | |
| Innate immunity; acute phase | |||||
| Complement C1q subcomponent γ | Q02105 | 2.04/5 | 1.18/7 | 3.97/11 | 1.50/5 |
| Complement factor B | P04186 | 3.19/6 | 0.76/4 | 0.89/6 | 0.79/3 |
| Serum amyloid A1/2 | P05366 | 0.79/15 | 64.03/17 | 0.89/3 | |
| P05367 | |||||
| Serum amyloid A3 | Q62201 | 42.22/4 | 1.56/8 | 80.12/11 | 0.72/7 |
| Liver metabolic enzymes | |||||
| Cytochrome p450 1A2 | P00186 | 0.20/4 | 0.89/9 | ||
| Cytochrome p450 2E1 | Q05421 | 0.25/5 | 0.82/4 | ||
| Microsomal GST; GST-3 | Q9CPU4 | 0.88/4 | 0.97/4 | 0.22/11 | |
| Alcohol dehydrogenase 1 | P00329 | 0.22/10 | 0.81/6 | ||
| Cytochrome p450 2C38 | P56656 | 0.12/33 | |||
| Hydroxysteroid sulfotransferase; Sth2; STA2 | P50236 | 0.10/8 | 27.88/70 | ||
| Miscellaneous | |||||
| Heat shock protein 1B; Hspa1b; HSP70 | P17879 | 1.96/14 | 0.70/4 | 11.18/9 | 1.30/14 |
| Prostaglandin-H2 d isomerase; PGHD | O09114 | 0.12/45 | 0.09/47 | ||
| Bone marrow stromal cell antigen 1; BST1; CD157 | Q64277 | 0.93/3 | 0.74/11 | 36.90/113 | |
| Apolipoprotein M; apoM | Q9Z1R3 | 0.29/2 | 0.79/5 | ||
| Transferrin | Q92111 | 0.25/12 | 1.09/11 | 0.88/5 | 1.03/4 |
| Superoxide dismutase 1 | P08228 | 1.23/3 | 1.61/8 | 0.30/11 | 0.88/9 |
| B-cell differentiation antigen CD72 | P21855 | 0.40/2 | 1.11/7 | 3.73/16 | |
| Sialophorin; CD43 | P15702 | 1.01/3 | 0.71/10 | 3.68/21 | |
| Mitochondrial superoxide dismutase 2 | Q8VEM5 | 3.05/7 | 1.13/5 | 0.93/3 | 0.92/15 |
| Growth arrest and DNA damage-inducible protein 45 | P48316 | 0.89/4 | 1.41/5 | 4.83/6 | 9.65 15 |
a
UNIPROT, protein database of the European Bioinformatics Institute.
b
Ratio of expression in mRNA of vehicle-treated mice on days, 0 and 8 p.i.
c
Ratio of expression in mRNA of infected mice on day 8 p.i. treated with vehicle (−T) or testosterone (+T).
d
ECM, extracellular matrix.