Table 1. Hematological parameters are unchanged by ERFE ablation in Tmprss6^−/− mice.

RBC was increased while hemoglobin, hematocrit and MCV were decreased in Tmprss6^−/− mice compared to WT mice at 6 weeks of age. Ablation of Erfe in Tmprss6^−/− mice did not result in any change in hematological parameters compared to Tmprss6^−/− animals at 6 weeks of age (two-tailed Student t-test comparison of Erfe^−/− Tmprss6^−/− and Erfe^+/− Tmprss6^−/− to Erfe^+/+ Tmprss6^−/−). None of the CBC parameters were statistically different by gender or by genotype when comparing Erfe^−/− Tmprss6^−/− and Erfe^+/− Tmprss6^−/− to Erfe^+/+ Tmprss6^−/− by two-way ANOVA. Data shown are means ± SEM and were compared for each group to control WT mice by two-tailed Student t-test (n=6 to 16 per group, gender combined).

	N	RBC (M/μL)	HB (g/dL)	HCT (%)	MCV (fL)
Males
WT	4	9.4 ± 0.2	16.3 ± 0.5	45.7 ± 1.2	48.6 ± 0.2
WT phlebotomized	3	8.0** ± 0.1	13.9*± 0.4	38.7**± 0.4	48.3 ± 0.7
Erfe +/+ Tmprss6 −/−	5	10.3 ± 0.4	8.4*** ± 0.3	34.9***± 1.2	34.0** ± 0.4
Erfe +/− Tmprss6 −/−	12	10.7* ± 0.3	8.9*** ± 0.2	35.7*** ± 0.7	33.5** ± 0.4
Erfe −/− Tmprss6 −/−	5	10.8* ± 0.5	9.0*** ± 0.4	36.6* ± 1.3	34.0*** ± 0.2
Females
WT	4	9.2 ± 0.1	16.3 ± 0.4	44.3 ± 0.6	48.1 ± 0.2
WT phlebotomized	3	6.8*** ± 0.3	11.8*** ± 0.4	33.3*** ± 1.1	48.6 ± 0.2
Erfe +/+ Tmprss6 −/−	5	11.4** ± 0.4	9.4*** ± 0.3	38.6** ± 1.1	33.9*** ± 0.2
Erfe +/− Tmprss6 −/−	4	10.2* ± 0.4	8.7*** ± 0.5	34.8*** ± 1.1	34.0*** ± 0.4
Erfe −/− Tmprss6 −/−	6	11.3*** ± 0.3	9.7** ± 0.2	37.6*** ± 0.9	33.3*** ± 0.1

^***P < .001,

^**P < .01,

^*P < .05.