TABLE 4.
Genes (108) that are upregulated 4 h following K. pneumoniae infection and are unique to C57BL/6 mice
| Affymetrix probe ID | Gene symbol | GenBank accession no. | Mean change (fold) in C57BL/6 mice at 4 vs 0 h | Results in C3H/HeN mice at 4 vs 0 ha | Description |
|---|---|---|---|---|---|
| 102712_at | Saa3 | X03505 | 47.8 | A | Serum amyloid A3 |
| 94769_at | Mmp8 | U96696 | 29.4 | A | Matrix metalloproteinase 8 |
| 92948_at | Csf2 | X03020 | 19.3 | A | Colony-stimulating factor 2 (granulocyte macrophage) |
| 93858_at | Cxcl10 | M33266 | 17.1 | A | Chemokine (C-X-C motif) ligand 10 |
| 160564_at | Lcn2 | X81627 | 11.8 | NS | Lipocalin 2 |
| 95951_at | Clecsf8 | AF061272 | 11.2 | A | C-type lectin |
| 96515_at | Il4i | U70430 | 10.9 | A | IL-4 induced 1 |
| 95303_at | EST | AA144469 | 10.9 | Chg | IMAGE-597616 |
| 103465_f_at | Saa2 | U60438 | 9.3 | A | Serum amyloid A2 |
| 98774_at | Irg1 | L38281 | 9.25 | A | Immunoresponsive gene 1 |
| 96153_at | Ngp | L37297 | 8.8 | A | Neutrophilic granule protein |
| 94688_at | Mad | X83106 | 8.2 | A | Max dimerization protein |
| 97106_at | Map3k8 | D13759 | 8.2 | A | Mitogen-activated protein kinase kinase kinase 8 |
| 97783_at | Ccl17 | AJ242587 | 8.1 | A | Chemokine (C-C motif) ligand 17 |
| 161689_f_at | Il1r2 | AV223216 | 7.6 | NS | IL-1 receptor, type II |
| 162206_f_at | Socs3 | AV374868 | 7.3 | NS | Suppressor of cytokine signaling 3 |
| 104692_at | Selp | M72332 | 7.2 | A | Selectin, platelet |
| 92217_s_at | gp49b | V05265 | 7.2 | NS | Glycoprotein 49B |
| 99333_at | Sele | M80778 | 5.9 | A | Selectin, endothelial cell |
| 102816_at | Serpina 3m | X69832 | 5.9 | A | Serine protease inhibitor, clade A, member 3M |
| 94761_at | Ccl7 | X70058 | 5.0 | A | Chemokine (C-C motif) ligand 7 |
| 95808_g_at | GCSFR | V05894 | 4.9 | A | Colony-stimulating factor, granulocyte receptor pseudogene |
| 101912_at | EST | AI01967 | 4.6 | Chg | IMAGE-1364952 |
| 101800_at | Fprrs2 | AF071180 | 4.6 | NS | N-formylpeptide receptor-like 2 gene |
| 160489_at | Tnfaip2 | L24118 | 4.4 | NS | TNF-α-induced protein 2 |
| 103839_at | SPHK1a | AF068748 | 4.1 | A | Spingosine kinase |
| 160608_at | Rab20 | AI854462 | 3.8 | A | RAB20, member RAS oncogene family |
| 92276_at | Map3k6 | AB021861 | 3.5 | A | Mitogen-activated protein kinase kinase kinase 6 |
| 102114_f_at | Angpt14 | AI326963 | 3.5 | A | Angiopoietin-like 4 |
| 97197_r_at | EST | C78850 | 3.5 | NS | Unknown |
| 96704_at | Sfn | AF058798 | 3.4 | A | Stratifin |
| 101820_at | Ly-6G1 | X70920 | 3.4 | A | Neurotoxin homologue, exons 1-3 |
| 102798_at | Adm | U77630 | 3.4 | Chg | Adrenomedullin |
| 102239_at | Bcl3 | M90397 | 3.3 | A | B-cell leukemia/lymphoma 3 |
| 102921_s_at | Tnfrsf6 | M83649 | 3.3 | Chg | TNF receptor superfamily, member 6 |
| 94147_at | Serpine1 | M33960 | 3.3 | Chg | Serine (or cysteine) proteinase inhibitor, clade E, member 1 |
| 93869_s_at | Ald | U23781 | 3.3 | Fld<2 | Hematopoietic-specific early response A1-d gene |
| 160894_at | Cebpd | X61800 | 3.2 | NS | CCAAT/enhancer binding protein (C/EBP), delta |
| 102707_f_at | Serpina 3C | X61597 | 3.2 | A | Serine (or cysteine) proteinase inhibitor, clade A, member 3C |
| 104333_at | G7e | U69488 | 3.2 | NS | Viral envelope-like |
| 98299_s_at | Slfn3 | AF09997 | 3.2 | A | Schlafen 3 |
| 104374_at | Spi2/eb4 | M64086 | 3.1 | Chg | Spi2 proteinase inhibitor |
| 102337_s_at | FCRII | M31312 | 3.1 | A | Mouse beta Fc receptor type II |
| 94137_at | I18rb | L13239 | 3.0 | A | IL-8 receptor, beta |
| 102021_at | Il4ra | M27960 | 3.0 | Fld<2 | IL-4 receptor, alpha |
| 92472_f_at | Slfn2 | AF099973 | 2.9 | NS | Schlafen 2 |
| 102860_at | Spi2/eb1 | M64085 | 2.9 | A | Spi2 prot |
| 93037_i_at | Anxa1 | M69260 | 2.9 | Chg | Annexin A1 |
| 94747_at | Csf2rb1 | M34397 | 2.9 | A | IL-3 receptor, beta chain 1 |
| 93411_at | EST | AI52789 | 2.8 | Chg | IMAGE-1478197 |
| 95784_at | Pira | U96682 | 2.8 | A | Paired immunoglobulin like receptor A1 |
| 97949_at | Fgl2 | M16238 | 2.7 | Fld<2 | Fibrinogen-like protein 2 |
| 97203_at | Mlp | X61399 | 2.7 | NS | MARCKS-like protein |
| 96119_s_at | Angpt14 | AA797604 | 2.7 | NS | Angiopoietin-like 4 |
| 102313_at | Gch | L09737 | 2.6 | NS | GTP cyclohydrolase 1 |
| 92925_at | Cebpb | M61007 | 2.6 | NS | CCAAT/enhancer binding protein (C/EBP), beta |
| 93058_at | Elf-1A | AF026481 | 2.6 | NS | Eukaryotic translation initiation factor 1A |
| 93860_i_at | M17327 | 2.6 | NS | Mouse endogenous murine leukemia virus modified polytropic provirus | |
| 160353_i_at | Mapkapk2 | X76850 | 2.5 | NS | Map kinase-activated protein kinase 2 |
| 96042_at | Sod2 | L35528 | 2.5 | NS | Superoxide dismutase 2, mitochondrial |
| 99957_at | Mmp9 | X72795 | 2.5 | A | Matrix metalloproteinase 9 |
| 160645_at | Birc2 | U88908 | 2.5 | NS | Baculoviral IAP repeat-containing 2 |
| AFFX-MurFAS_at | Tnfrsf6 | M83649 | 2.5 | NS | TNF receptor superfamily, member 6 |
| 102362_i_at | Junb | U20735 | 2.5 | NS | Jun-B oncogene |
| 104406_at | Ptges | AI060798 | 2.4 | A | Prostaglandin E synthase |
| 160646_at | Gsr | AI851983 | 2.4 | NS | Glutathione reductase I |
| 94351_r_at | Nqo1 | U12961 | 2.4 | A | NAD(P)H dehydrogenase, quinine 1 |
| 97525_at | Gyk | U48403 | 2.4 | NS | Glycerol kinase |
| 97950_at | Xdh | X75129 | 2.4 | NS | Xanthine dehydrogenase |
| 102264_at | Slfn1 | AF099972 | 2.4 | A | Schlafen 1 |
| 94556_at | Snx10 | A1746846 | 2.4 | NS | Sorting nexin 10 |
| 95785_s_at | Rab7 | Y13361 | 2.4 | NS | RAB7, member RAS oncogene family |
| 102957_at | Lcp2 | U20159 | 2.4 | NS | Lymphocyte cytosolic protein 2 |
| 95706_at | Lgals3 | X16834 | 2.4 | NS | Lectin galactose binding soluble 3 |
| 101464_at | Timp1 | V00755 | 2.4 | NS | Immunoresponsive gene 1 |
| 93328_at | Hdc | X57437 | 2.4 | NS | Histidine decarboxylase |
| 160583_at | Xlkd1 | AA880988 | 2.3 | NS | Extracellular link domain-containing 1 |
| 99384_at | Pim1 | M13945 | 2.3 | A | Proviral integration site 1 |
| 102806_g_at | Ceacam1 | M77196 | 2.3 | NS | CEA-related cell adhesion molecule 1 |
| 160975_at | EST | AI504338 | 2.3 | NS | IMAGE-963414 |
| 100328_s_at | Akr1b8 | U96684 | 2.3 | A | Ado-keto reductase family 1, member B8 |
| 92251_f_at | EST | AA960657 | 2.3 | A | IMAGE-2076183 |
| 103091_at | Relb | M83380 | 2.2 | A | Avian reticuloendotheliosis viral (v-rel) oncogene related B |
| 93202_at | Nt5e | L12059 | 2.2 | NS | 5′ nucleotidase, ecto |
| 101554_at | Nfkbia | U57524 | 2.2 | Fld<2 | Nuclear factor of kappa light chain gene enhancer in B cells inhibitor, alpha |
| 96562_at | Slc11a1 | L13732 | 2.2 | A | Solute carrier family 11 member 1 |
| 99461_at | Hcls1 | X84797 | 2.2 | A | Lyn substrate 1 |
| 100884_at | U04204 | 2.2 | Chg | Fibroblast growth factor regulated protein | |
| 101908_s_at | Bgp2 | AF101164 | 2.2 | A | Biliary glycoprotein 2 long isoform |
| 162198_f_at | EST | AV373027 | 2.2 | NS | Unknown |
| 94085_at | M34603 | 2.2 | NS | Proteoglycan secretory granule | |
| 160359_at | EST | AI746846 | 2.2 | NS | Unknown |
| 96295_at | Psat1 | AW122030 | 2.1 | NS | Phosphoserine aminotransferase 1 |
| 102310_at | Cc122 | AF052505 | 2.1 | A | Chemokine (C-C motif) ligand 22 |
| 99387_at | Fpr1 | L22181 | 2.1 | NS | Formyl peptide receptor 1 |
| 161132_at | Sce1 | AA727482 | 2.1 | NS | Screllin |
| 99160_s_at | EST | AW227647 | 2.1 | NS | IMAGE-2654099 |
| 93624_at | EST | AI225296 | 2.1 | NS | Unknown |
| 100397_at | Tryrobp | AF024637 | 2.1 | NS | TYRO protein tyrosine kinase binding protein |
| 160108_at | Nupr1 | AI852641 | 2.1 | NS | Nuclear protein 1 |
| 160387_at | EST | AI853900 | 2.1 | NS | Unknown |
| 94821_at | Xbp1 | AW123880 | 2.1 | Fld<2 | X-box binding protein 1 |
| 101923_at | Pla2g7 | U34277 | 2 | A | Phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
| 101996_at | Ptpn2 | M80739 | 2.0 | Chg | Protein tyrosine phosphatase, nonreceptor type 2 |
| 94224_s_at | Ifi205 | M74123 | 2.0 | Chg | Interferon-activated gene 205 |
| 92877_at | Tgfbi | L19932 | 2.0 | NS | Transforming growth factor, beta induced |
| 97825_at | Perp-pending | AI854029 | 2.0 | A | p53 apoptosis effector related to Pmp22 |
a
Fold changes were not shown for those genes that had detection calls of absent (A) in the 4-h treated group; that had an inappropriate change call (Chg), such that there was not a call of increased or marginally increased in greater than half of the comparisons made; that had a fold change less than 1 (Fld<2); or that were not determined to be significant (NS) by parametric and nonparametric tests, with a P cutoff value of ≤0.05.