TABLE 2.
Primers used in this study
| Gene | GenBank accession no. | Product (bp) | Annealing temp (°C) | Oligonucleotide sequencesb |
|---|---|---|---|---|
| IL-1βa | M26295 | 354 | 60 | TACAACAAGAGCTTCCGGCA |
| GGCCACAGGTATCTTGTCGT | ||||
| TNF-αa | M12845 | 252 | 60 | AGCCCACGTAGTAGCAAACCC |
| TTGATGGCAGAGAGGAGGTTGA | ||||
| IL-6 | AF169176 | 399 | 60 | TCCGGATGTATCTCGAGCAC |
| CTAACGCTCATCTTCCTAGTTTCG | ||||
| IL-10 | AF068058 | 577 | 55 | TACTAGTCTCTGCTATGTTGCCTGGTCTT |
| ATGATCAACTGATGTCCTAGACTCTAGCCGA | ||||
| IL-8 | M58021 | 989 | 55 | GGCCGTAATGGAAGTAAACGT |
| GGAACTCCTTGCTAATAAGGCC | ||||
| GAPDHa | L23961 | 293 | 60 | TCACCATCTTCCAGGAGCGA |
| CACAATGCCGAAGTGGTCGT |
a
Data are from Reno et al. (32).
b
Sense and antisense primers.