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. Author manuscript; available in PMC: 2017 Apr 25.
Published in final edited form as: Mucosal Immunol. 2016 Oct 12;10(3):705–715. doi: 10.1038/mi.2016.90

Figure 6.

Figure 6

GM-CSF is required for optimal dendritic cell function and Th2 priming. C57BL/6 mice were treated with rIgG2a or a neutralizing anti-GM-CSF antibody, five hours prior to intranasal administration with A647-CRA. The draining lymph node was collected 24 hours later and cells were analyzed by flow cytometry to assess the uptake of CRA and maturation of DCs. DCs were gated as CD11c+ and MHCII+. (a) Total number of A647+ DCs and percent of A647+ DCs within the DC population. (b) Mean fluorescent intensity (MFI) of costimulatory molecule CD80 on A647+ DCs. Data represent combined data of two independent experiments, mean ± SEM (n = 9 mice per group). (c) On days 0–2, C57BL/6 mice were sensitized with CRA. On day 3, the lung draining lymph node was collected and cells were restimulated with CRA. On day 7, culture supernatant was collected and concentration of IL-4 and IL-13 were determined by ELISA. Data represent mean ± SEM of one experiment (n = 3–5 mice per group) and are representative of two independent experiments. Comparisons were made using unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001.