(A, top to bottom) A typical time course of temperature, length change, tension and x-ray shutter opening signal during a run of the experimental protocol in a bundle of three muscle fibers (from top to bottom, (A)). Step stretches were applied to the bundle immediately after each x-ray frame to measure instantaneous stiffness. (B and C) Expanded views of the parts of the record indicated by ellipses (B) and (C), respectively, in (A).