Figure 2.

Off-meridional x-ray intensity plotted against meridional reciprocal spacing was collected from 43 runs of the experimental protocol in six bundles of muscle fibers during isometric contraction and steady shortening (solid lines, see color code). The intensity was integrated along equatorial direction at reciprocal radii from 0.005 to 0.15 nm⁻¹. Some myosin (M) and actin (A) layer lines are labeled. Inset: intensity profiles in the region of the M1 and A1 layer lines, and their breakdown into their Gaussian components, namely, the M1 (dash-dot lines) and A1 (dashed lines) layer lines during isometric contraction and shortening (see color code). To see this figure in color, go online.