Figure 2.

BK channel is critical in substrate stiffness regulation of L-type Ca²⁺ channel in ventricular myocytes. (A) Representative whole-cell recordings of Ba²⁺ currents from cells cultured on the soft and stiff substrates after treatment with 100 nM IBTX for 10 min. (B) Mean I-V relationships from 12 cells in three preparations for each case. (C) Representative whole-cell recordings of Ca²⁺ currents from cells cultured on the soft and stiff substrates after treatment with 100 nM IBTX for 10 min. (D) Mean I-V relationships from 10 cells in three preparations for each case. (E) Voltage dependence of L-type channel activation determined with tail currents from 12 cells cultured on the soft and stiff substrates after treatment with 100 nM IBTX for 10 min. (F) Voltage dependence of L-type channel inactivation from 12 cells in three preparations on the soft and stiff substrates after treatment with 100 nM IBTX for 10 min.