Figure 7.

Increased BK channel current and decreased L-type Ca²⁺ channel current in ventricular myocytes on stiff substrate after treatment with low-dose blebbistatin. (A) Representative whole-cell recordings of outward K⁺ currents from cells after treatment with 5 μM blebbistatin on the stiff substrate and cells on the soft substrate before (upper panels), and 10 min after, application of 100 nM IBTX (middle panels) and IBTX-sensitive currents (bottom panels). (B) Mean I-V relationships from 10 cells in three preparations for each case. (C) Representative whole-cell recordings of Ba²⁺ currents from cells on the stiff substrates after treatment with low-dose blebbistatin and cells on the soft substrate without blebbistatin treatment. (D) Mean I-V relationships from 11 cells in three preparations for each case.