FIG. 1.

Structural and biological properties of LL-37 and LL-37B. (A) CD spectra of LL-37 and LL-37B. LL-37 (4 μM) and LL-37B (4.3 μM) were incubated in 10 mM Tris-HCl buffer (pH 7.4) in the presence and absence of 1 mM POPC-POPG (1:1) liposomes. PC, POPC; PG, POPG. (B) A549 cells were incubated with LL-37 or LL-37B for 4 h, the RNA was harvested, and total RNA was used to make labeled cDNA probes (biotin or fluorescein), which were hybridized onto microarrays spotted with 14,000 human genes in duplicate and labeled as described in Materials and Methods. RLS images were quantified, the densities were normalized, and the average intensities for LL-37 were plotted against those of LL-37B on a log scale. The plot represents averages of three biological replicates and two technical replicates for each biological replicate.