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. 2017 Apr 13;8:432. doi: 10.3389/fimmu.2017.00432

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Copyright © 2017 Morgan and Davis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glucocorticoids prevent cytokine-induced cell death of primary human NK (pNK) cells. (A–H) pNK cells and (I–J) freshly isolated pNK cells were treated with a vehicle control or Dex (100 nM) for 1 h, then cultured for 5 days with either (A) interleukin-12 (IL-12) (10 ng/ml), (B) interleukin-18 (IL-18) (100 ng/ml), (C,I) IL-2 (200 U/ml), (D) interleukin-15 (IL-15) (5 ng/ml), (E) IL-12 + IL-15, (F) IL-12 + IL-18, (G) IL-12 + IL-15 + IL-18, or (H,J) IL-2 + IL-12. Natural killer cell survival was assessed by flow cytometry. Left panels display representative dot plots of forward scatter (FSC) against Live/Dead stain, Zombie Dead Cell Marker (DCM) for pNK cells. Right panels show percentage of dead pNK cells (mean ± SD) for (A) six, (B) three, (C,D) seven, (E) three, (F,G) four, (H) six, or (I,J) four independent experiments. Samples are compared by unpaired, two-tailed Student’s t-test (*p < 0.05; **p < 0.005; ***p < 0.0005; ns, not significant).

Glucocorticoids prevent cytokine-induced cell death of primary human NK (pNK) cells. (A–H) pNK cells and (I–J) freshly isolated pNK cells were treated with a vehicle control or Dex (100 nM) for 1 h, then cultured for 5 days with either (A) interleukin-12 (IL-12) (10 ng/ml), (B) interleukin-18 (IL-18) (100 ng/ml), (C,I) IL-2 (200 U/ml), (D) interleukin-15 (IL-15) (5 ng/ml), (E) IL-12 + IL-15, (F) IL-12 + IL-18, (G) IL-12 + IL-15 + IL-18, or (H,J) IL-2 + IL-12. Natural killer cell survival was assessed by flow cytometry. Left panels display representative dot plots of forward scatter (FSC) against Live/Dead stain, Zombie Dead Cell Marker (DCM) for pNK cells. Right panels show percentage of dead pNK cells (mean ± SD) for (A) six, (B) three, (C,D) seven, (E) three, (F,G) four, (H) six, or (I,J) four independent experiments. Samples are compared by unpaired, two-tailed Student’s t-test (*p < 0.05; **p < 0.005; ***p < 0.0005; ns, not significant).